2V50
The Missing Part of the Bacterial MexAB-OprM System: Structural determination of the Multidrug Exporter MexB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-26 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 125.050, 134.580, 151.020 |
| Unit cell angles | 86.99, 69.70, 88.16 |
Refinement procedure
| Resolution | 49.760 - 3.000 |
| R-factor | 0.243 |
| Rwork | 0.242 |
| R-free | 0.28700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j8s |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.965 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.040 | 0.700 |
| Number of reflections | 176904 | |
| <I/σ(I)> | 15.26 | 1.7 |
| Completeness [%] | 95.7 | 87 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.5 | 30% PEG400, 50MM NA-ACETATE, 230MM NACL, pH 4.5 |
