2V03

High resolution structure and catalysis of an O-acetylserine sulfhydrylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	76.615, 76.615, 209.787
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 1.330
R-factor	0.158
Rwork	0.158
R-free	0.17200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.711
Refinement software	REFMAC (5.3.0022)

Data quality characteristics

	Overall
Low resolution limit [Å]	63.000
High resolution limit [Å]	1.330
Rmerge	0.060
Number of reflections	83156
Completeness [%]	98.6
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.2		pH 5.2