2UZ9
Human guanine deaminase (guaD) in complex with zinc and its product Xanthine.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-01-17 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.735, 85.713, 78.960 |
| Unit cell angles | 90.00, 116.49, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.172 |
| Rwork | 0.169 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2i9u |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.366 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.100 | 0.510 |
| Number of reflections | 24361 | |
| <I/σ(I)> | 13.3 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
