2UYZ

Non-covalent complex between Ubc9 and SUMO1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-06-18
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	49.481, 35.030, 72.915
Unit cell angles	90.00, 93.41, 90.00

Refinement procedure

Resolution	50.000 - 1.400
R-factor	0.143
Rwork	0.141
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u9a 2bf8
RMSD bond length	0.014
RMSD bond angle	1.556
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.060	0.230
Number of reflections	49557
<I/σ(I)>	6.3	1.6
Completeness [%]	99.2	99.3
Redundancy	3.5	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5		16.5 % (W/V) PEG3350 100 MM BISTRIS PH 5.5 15 % (W/V) GLYCEROL