2UXN

Structural Basis of Histone Demethylation by LSD1 Revealed by Suicide Inactivation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-14
Detector	CUSTOM
Spacegroup name	I 2 2 2
Unit cell lengths	119.949, 178.646, 234.930
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.000 - 2.720
R-factor	0.242
Rwork	0.241
R-free	0.27200
Structure solution method	OTHER
RMSD bond length	0.008
RMSD bond angle	1.205
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.570	2.710
High resolution limit [Å]	2.690	2.690
Rmerge	0.050	0.680
Number of reflections	69440
<I/σ(I)>	36.6	1.8
Completeness [%]	99.9	98.5
Redundancy	9.9	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6		PROTEIN: 25 MM HEPES, PH 7.4, 200 MM SODIUM CHLORIDE, 1 MM PMSF, AND 5 MM DTT RESERVOIR: 0.8 M LITHIUM SULFATE, 0.8 M AMMONIUM SULFATE, 0.4 M SODIUM CHLORIDE, 0.1 M SODIUM CITRATE, PH 5.6, AND 10 MM DTT