2UV3
Structure of the signal-regulatory protein (SIRP) alpha domain that binds CD47.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8856, 0.9783, 0.9079, 0.9792 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.831, 56.266, 82.245 |
| Unit cell angles | 90.00, 114.90, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.207 |
| Rwork | 0.204 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | STRUCTURE DETERMINED BY MAD IN SPACE GROUP P21212 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 0.280 |
| Number of reflections | 441122 | |
| <I/σ(I)> | 22.1 | 3.8 |
| Completeness [%] | 99.2 | 98 |
| Redundancy | 3.7 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 2.4 M AMMONIUM SULPHATE, 0.1 M MES, PH 6.0 |
