Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 87 |
Detector technology | AREA DETECTOR |
Detector | SIEMENS HI-STAR |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.000, 78.700, 128.600 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.800 |
R-factor | 0.186 * |
Rwork | 0.186 |
RMSD bond length | 0.011 |
RMSD bond angle | 15.000 * |
Data scaling software | XSCALIBRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | |
High resolution limit [Å] | 1.800 | 1.800 * |
Rmerge | 0.046 | 0.230 * |
Total number of observations | 146373 * | |
Number of reflections | 68170 | 7563 * |
Completeness [%] | 97.0 | 87 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 20 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 5 (mM) | |
3 | 1 | drop | UDP-phenol | 2.5 (mM) | |
4 | 1 | drop | PEG8000 | 10 (%) | |
5 | 1 | drop | HEPES | 25 (mM) | |
6 | 1 | drop | 250 (mM) | ||
7 | 1 | reservoir | PEG8000 | 20 (%) | |
8 | 1 | reservoir | HEPES | 50 (mM) | |
9 | 1 | reservoir | 500 (mM) |