2RKJ
Cocrystal structure of a tyrosyl-tRNA synthetase splicing factor with a group I intron RNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-12-07 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 231.063, 123.525, 235.231 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 47.880 - 4.500 |
| Rwork | 0.235 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1Y42 1y0q |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.660 |
| High resolution limit [Å] | 4.500 | 4.500 |
| Rmerge | 0.094 | 0.394 |
| Number of reflections | 66194 | |
| <I/σ(I)> | 11 | 1.86 |
| Completeness [%] | 82.8 | 42.5 |
| Redundancy | 3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1M cacodylate, 1.8 M ammonium sulfate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | cacodylate | ||
| 2 | 1 | 1 | ammonium sulfate | ||
| 3 | 1 | 2 | cacodylate | ||
| 4 | 1 | 2 | ammonium sulfate |
