1Y0Q

Crystal structure of an active group I ribozyme-product complex

Summary for 1Y0Q

• Entry DOI: 10.2210/pdb1y0q/pdb
• Descriptor: Group I ribozyme, 5'-R(*GP*CP*UP*U)-3', SPERMINE (FULLY PROTONATED FORM), ... (5 entities in total)
• Functional Keywords: ribozyme, rna-splicing, guanosine-binding, rna-folding, rna
• Biological source: Staphylococcus phage Twort; More
• Total number of polymer chains: 2
• Total formula weight: 75550.36

Authors

Golden, B.L.,Kim, H.,Chase, E. (deposition date: 2004-11-16, release date: 2004-12-21, Last modification date: 2024-02-14)

Primary citation

Golden, B.L.,Kim, H.,Chase, E.
Crystal structure of a phage Twort group I ribozyme-product complex
Nat.Struct.Mol.Biol., 12:82-89, 2005

PubMed Abstract: Group I introns are catalytic RNAs capable of orchestrating two sequential phosphotransesterification reactions that result in self-splicing. To understand how the group I intron active site facilitates catalysis, we have solved the structure of an active ribozyme derived from the orf142-I2 intron from phage Twort bound to a four-nucleotide product RNA at a resolution of 3.6 A. In addition to the three conserved domains characteristic of all group I introns, the Twort ribozyme has peripheral insertions characteristic of phage introns. These elements form a ring that completely envelops the active site, where a snug pocket for guanosine is formed by a series of stacked base triples. The structure of the active site reveals three potential binding sites for catalytic metals, and invokes a role for the 2' hydroxyl of the guanosine substrate in organization of the active site for catalysis.

PubMed: 15580277
DOI: 10.1038/nsmb868

Experimental method

X-RAY DIFFRACTION (3.6 Å)