2RKG
HIV-1 PR resistant mutant + LPV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-21 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 61 |
| Unit cell lengths | 62.010, 62.010, 83.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.090 - 1.800 |
| R-factor | 0.19523 |
| Rwork | 0.191 |
| R-free | 0.24869 |
| Structure solution method | SAD |
| Starting model (for MR) | 1u8g |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.385 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 16989 | |
| <I/σ(I)> | 34.8 | 2.8 |
| Completeness [%] | 99.1 | 94.1 |
| Redundancy | 5.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | reservoir: 0.1M MES pH 5.5, 0.5M Ammonium Sulfate; drops: 2ul protein + 1ul reservoir Cpr=8mg/ml, 5-fold molar excess of LPV microseeding, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
