2RJ3

Crystal Structure of the Uridine Phosphorylase from Salmonella Typhimurium in Complex with Uracil and Phosphate Ion at 2.49A Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Collection date	2005-09-25
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	89.000, 124.260, 133.960
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.980 - 2.510
Rwork	0.221
R-free	0.25652
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2oec
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	26.840
High resolution limit [Å]	2.490
Number of reflections	47321

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	VAPOR DIFFUSION, HANGING DROP, temperature 297K