2RGN
Crystal Structure of p63RhoGEF complex with Galpha-q and RhoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Detector technology | CCD |
| Collection date | 2007-04-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9993 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.187, 68.056, 138.018 |
| Unit cell angles | 80.87, 85.16, 87.09 |
Refinement procedure
| Resolution | 20.000 - 3.500 |
| Rwork | 0.243 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bcj (Galpha-Q) 1lb1 (RhoA; Dbs for p63) |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.139 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.087 | 0.531 |
| Number of reflections | 29299 | |
| <I/σ(I)> | 5.7 | 1.67 |
| Completeness [%] | 97.1 | 98.1 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 100 mM HEPES pH 6.5, 14.5% PEG 3350, 400 mM KCl. Drops were microseeded to obtain large crystals, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
