2RFJ
Crystal structure of the bromo domain 1 in human bromodomain containing protein, testis specific (BRDT)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-09-22 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97866 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 42.935, 92.679, 100.107 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.063 - 2.050 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nxb |
RMSD bond length | 0.016 |
RMSD bond angle | 1.517 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.063 | 2.160 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.129 | 0.681 |
Total number of observations | 8196 | |
Number of reflections | 24939 | |
<I/σ(I)> | 4.6 | 1 |
Completeness [%] | 96.4 | 77.6 |
Redundancy | 6.4 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.20M Na/K tartrate, 20.0% PEG 3350, 10.0% Ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |