2RFB
Crystal Structure of a Cytochrome P450 from the Thermoacidophilic Archaeon Picrophilus Torridus
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL1-5 |
Synchrotron site | SSRL |
Beamline | BL1-5 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-12-17 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.7400, 1.6475 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.790, 168.460, 88.230 |
Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
Resolution | 48.510 - 2.500 |
R-factor | 0.21434 |
Rwork | 0.211 |
R-free | 0.26844 |
Structure solution method | MAD |
RMSD bond length | 0.017 |
RMSD bond angle | 1.619 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.510 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.051 | 0.661 |
Number of reflections | 48305 | |
<I/σ(I)> | 31.1 | 2 |
Completeness [%] | 98.1 | 95.1 |
Redundancy | 3.5 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 2M ammonium sulfate, 0.1M HEPES, 2% polyethylene glycol 400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |