2RDJ
Snapshots of a Y-family DNA polymerase in replication: Dpo4 in apo and binary/ternary complex forms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 97.993, 102.510, 106.109 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.110 - 2.200 |
| R-factor | 0.221 |
| Rwork | 0.210 |
| R-free | 0.25600 |
| Starting model (for MR) | 1jx4 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.502 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.055 | 0.360 |
| Number of reflections | 51948 | |
| <I/σ(I)> | 18.1 | |
| Completeness [%] | 97.5 | 99.7 |
| Redundancy | 2.8 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 294 | 10-15% PEG3350, 0.2M calcium acetate, 0.1M HEPES pH 7.0, 5% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG3350 | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | PEG3350 | ||
| 6 | 1 | 2 | calcium acetate | ||
| 7 | 1 | 2 | HEPES | ||
| 8 | 1 | 2 | glycerol |
