Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-01-16 |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.310, 70.310, 187.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.440 - 2.800 |
| R-factor | 0.23607 |
| Rwork | 0.233 |
| R-free | 0.26655 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fuk |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.244 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.107 | 0.820 |
| Number of reflections | 12185 | |
| <I/σ(I)> | 15.65 | 2.59 |
| Completeness [%] | 100 | |
| Redundancy | 8.8 | 9.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M Bis-Tris propane, 2.5 M Ammonium sulfate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
