2R52

Crystal structure analysis of Bone Morphogenetic Protein-6 (BMP-6)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-01-16
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 31 2 1
Unit cell lengths	97.655, 97.655, 85.048
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	59.980 - 2.500
R-factor	0.24
Rwork	0.235
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1BMP BMP-7
RMSD bond length	0.007
RMSD bond angle	1.152
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.980	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	16706
<I/σ(I)>	13.4	3.2
Completeness [%]	99.4	99.9
Redundancy	3.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4	293	20% 2-propanol, 0.1M sodium citrate pH 4.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K