2R3V
The Biochemical and Structural Basis for Feedback Inhibition of Mevalonate Kinase and Isoprenoid Metabolism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-08-25 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 102.030, 78.200, 109.450 |
| Unit cell angles | 90.00, 113.33, 90.00 |
Refinement procedure
| Resolution | 29.370 - 2.500 |
| R-factor | 0.239 |
| Rwork | 0.239 |
| R-free | 0.28200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kvk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MERLOT |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.370 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 46347 | |
| <I/σ(I)> | 17.7 | 3.2 |
| Completeness [%] | 84.1 | 44.3 |
| Redundancy | 3.2 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 277 | MES buffer, PEG5k MME, ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
