2R35
Crystal structure of RB human arg-insulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-03-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 80.522, 80.522, 38.329 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.790 - 2.080 |
| R-factor | 0.2431 |
| Rwork | 0.237 |
| R-free | 0.29842 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1trz |
| RMSD bond length | 0.108 |
| RMSD bond angle | 7.670 |
| Data reduction software | AUTOMAR |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.240 | 0.650 |
| Number of reflections | 4169 | |
| <I/σ(I)> | 2.2 | 0.8 |
| Completeness [%] | 70.3 | 52 |
| Redundancy | 1.3 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 293 | Sodium Citrate, Acetone, Rubidium Chloride, pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
