2R2L

Structure of Farnesyl Protein Transferase bound to PB-93

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	95
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	171.614, 171.614, 69.155
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.230
Rwork	0.189
R-free	0.22100
RMSD bond length	0.006
RMSD bond angle	1.260
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.270
High resolution limit [Å]	2.230	2.230
Rmerge	0.061	0.308
Number of reflections	56633
<I/σ(I)>	17.3	4.2
Completeness [%]	100.0	100
Redundancy	4.2	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP