2R2I

Myristoylated Guanylate Cyclase Activating Protein-1 with Calcium Bound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-06-07
Detector	ADSC QUANTUM 4
Wavelength(s)	1.5498
Spacegroup name	P 41 21 2
Unit cell lengths	73.333, 73.333, 73.229
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.930 - 2.000
R-factor	0.218
Rwork	0.218
R-free	0.25100
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	13602
Completeness [%]	96.7	98.2
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	24% PEG 20,000, 0.4 M potassium nitrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	24% PEG 20,000, 0.4 M potassium nitrate, 0.1 M MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K