2R24
Human Aldose Reductase structure
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 298 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.066, 67.126, 47.862 |
| Unit cell angles | 90.00, 92.41, 90.00 |
Refinement procedure
| Resolution | 40.110 - 2.194 |
| R-factor | 0.2596 |
| Rwork | 0.257 |
| R-free | 0.29120 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.759 |
| Data reduction software | LAUEGEN |
| Data scaling software | LSCALE |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.110 |
| High resolution limit [Å] | 1.750 |
| Number of reflections | 46319 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 297 | pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 297K |
