2R09

Crystal Structure of Autoinhibited Form of Grp1 Arf GTPase Exchange Factor

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Collection date	2004-09-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9791, 0.97872, 1.1627
Spacegroup name	P 21 21 21
Unit cell lengths	83.599, 94.611, 115.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.900
R-factor	0.20558
Rwork	0.204
R-free	0.24106
Structure solution method	MAD
RMSD bond length	0.008
RMSD bond angle	1.074
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	1.950
Number of reflections	72844
Completeness [%]	99.9
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277.15	10-15% PEG 6000, 50 mM Tris, pH 8.0, 0.2 M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K