2R00
crystal structure of aspartate semialdehyde dehydrogenase II complexed with ASA from vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-C |
Synchrotron site | APS |
Beamline | 14-BM-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-09-29 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 122.376, 84.679, 114.976 |
Unit cell angles | 90.00, 102.07, 90.00 |
Refinement procedure
Resolution | 40.000 - 2.030 |
R-factor | 0.19319 |
Rwork | 0.191 |
R-free | 0.23237 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ys4 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.187 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 70525 | |
<I/σ(I)> | 27 | 5 |
Completeness [%] | 99.7 | 96.1 |
Redundancy | 3.6 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 0.1M sodium citrate pH 5.5, 27% PEG8000, 0.3M ammonium acetate, 5mM DTT, VAPOR DIFFUSION, HANGING DROP, temperature 293K |