2QZV
Draft Crystal Structure of the Vault Shell at 9 Angstroms Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2004-01-16 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.08 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 631.449, 464.724, 584.572 |
Unit cell angles | 90.00, 123.84, 90.00 |
Refinement procedure
Resolution | 200.000 - 9.000 |
Rwork | 0.615 |
Structure solution method | AB INITIO PHASING |
Starting model (for MR) | CRYO EM DENSITY |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | DM |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 200.000 | 9.500 |
High resolution limit [Å] | 9.000 | 9.000 |
Number of reflections | 101681 | |
<I/σ(I)> | 6.5 | 1.2 |
Completeness [%] | 98.1 | 95.5 |
Redundancy | 6.1 | 5.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.5% PEG 8000, 3% GLYCEROL, 0.05M NA MOPS, 0.04M MGCL2, 0.2% N-OCTYL-BETA-D-GLUCOPYRANOSIDE, INITIALLY SATURATED WITH CYCLOHEXANE, PH 6.8, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, PH 6.80 |