2QUK
Crystal structures of human tryptophanyl-tRNA synthetase in complex with ATP(putative)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 235.600, 235.600, 48.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.267 |
| Rwork | 0.267 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o5t |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.930 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.088 | 0.405 |
| Number of reflections | 20030 | |
| <I/σ(I)> | 21.1 | 2.6 |
| Completeness [%] | 99.1 | 93.9 |
| Redundancy | 7.6 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.2 | 277 | PEG3350, PEG1500, Na Citrate,, pH8.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
