2QUF
Crystal Structure of Transcription Factor AXXA-PF0095 from Pyrococcus furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.747, 93.484, 71.062 |
| Unit cell angles | 90.00, 102.44, 90.00 |
Refinement procedure
| Resolution | 32.530 - 2.800 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.28100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2plz |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.058 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.530 | 2.880 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 11712 | |
| Completeness [%] | 98.0 | 85 |
| Redundancy | 6.6 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.8 | 291 | 70mM Sodium Acetate, 30% Glycerol, pH 4.8, modified microbatch, temperature 291K |
