2QNC

Crystal structure of T4 Endonuclease VII N62D mutant in complex with a DNA Holliday junction

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-06-14
Detector	ADSC QUANTUM 4
Spacegroup name	P 43 21 2
Unit cell lengths	99.197, 99.197, 186.884
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.270 - 3.100
R-factor	0.258
Rwork	0.258
R-free	0.27800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	T4 ENDONUCLEASE VII-HETERODUPLEX COMPLEX
RMSD bond length	0.009
RMSD bond angle	1.200
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.270	3.220
High resolution limit [Å]	3.100	3.100
Number of reflections	17475
<I/σ(I)>	26.8	3.73
Completeness [%]	98.9	95.7
Redundancy	7.9	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.5	293	25 %(w/v) PEG 3350, 0.1 M Bis-Tris, pH 5.5, VAPOR DIFFUSION, temperature 293K, pH 5.50

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 3350
2	1	1	Bis-Tris
3	1	2	PEG 3350
4	1	2	Bis-Tris