2QN1

Glycogen Phosphorylase b in complex with asiatic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X13
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X13
Temperature [K]	293
Detector technology	CCD
Collection date	2007-02-15
Detector	MAR CCD 165 mm
Wavelength(s)	0.8088
Spacegroup name	P 43 21 2
Unit cell lengths	128.720, 128.720, 116.638
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	90.910 - 2.401
R-factor	0.18067
Rwork	0.179
R-free	0.20752
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	2prj
RMSD bond length	0.008
RMSD bond angle	1.129
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	90.910	2.440
High resolution limit [Å]	2.400	2.400
Number of reflections	38832
<I/σ(I)>	15.5	4.2
Completeness [%]	100.0	99.9
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	SMALL TUBES	6.7	289	10 mM Bes buffer, 3 mM DDT, pH 6.7, SMALL TUBES, temperature 289K