2QMR
Karyopherin beta2/transportin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 129.338, 168.892, 140.958 |
| Unit cell angles | 90.00, 93.18, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.26185 |
| Rwork | 0.261 |
| R-free | 0.28575 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2h4m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.124 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.950 |
| Rmerge | 0.083 |
| Number of reflections | 126673 |
| <I/σ(I)> | 24 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.2 M potassium formate, 0.1 M HEPES pH 7.5 and 10% Glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
