2QMJ
Crystral Structure of the N-terminal Subunit of Human Maltase-Glucoamylase in Complex with Acarbose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-19 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9175 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.970, 109.367, 109.271 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.780 - 1.900 |
| R-factor | 0.17923 |
| Rwork | 0.177 |
| R-free | 0.21516 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qly |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 82234 | |
| <I/σ(I)> | 15.3 | 3.4 |
| Completeness [%] | 98.7 | 96.2 |
| Redundancy | 7.6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 20% PEG 3350, 0.2M sodium sulfate, 4% 1,1,1,3,3,3-hexafluoro-2-propanol, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
