2QME
Crystal structure of human RAC3 in complex with CRIB domain of human p21-activated kinase 1 (PAK1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-01-31 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.54180 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 84.226, 53.480, 47.682 |
| Unit cell angles | 90.00, 93.45, 90.00 |
Refinement procedure
| Resolution | 47.620 - 1.750 |
| R-factor | 0.15114 |
| Rwork | 0.149 |
| R-free | 0.19177 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2g0n |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.620 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.053 | 0.343 |
| Number of reflections | 21448 | |
| <I/σ(I)> | 27.1 | 4 |
| Completeness [%] | 99.9 | 99.6 |
| Redundancy | 7.2 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Tris-HCl pH 8.5, 2 M (NH4)2S04, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
