2QL2
Crystal Structure of the basic-helix-loop-helix domains of the heterodimer E47/NeuroD1 bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-01 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 60.310, 169.540, 52.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.800 - 2.500 |
| Rwork | 0.248 |
| R-free | 0.28900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdy |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.154 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 169.546 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.076 | 0.770 |
| Total number of observations | 18434 | |
| Number of reflections | 18832 | |
| <I/σ(I)> | 5.7 | 1 |
| Completeness [%] | 97.7 | 94.8 |
| Redundancy | 7.2 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.6 | 291 | 0.1 mM Imidazole, 0.2 M ammonium citrate dibasic, and 22 % PEG 4000, pH 7.6, EVAPORATION, temperature 291K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Imidazole | ||
| 2 | 1 | 2 | Imidazole | ||
| 3 | 1 | 2 | ammonium citrate dibasic | ||
| 4 | 1 | 2 | PEG 4000 |
