2QKK
Human RNase H catalytic domain mutant D210N in complex with 14-mer RNA/DNA hybrid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-11 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.065, 176.200, 125.845 |
| Unit cell angles | 90.00, 90.22, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| Rwork | 0.212 |
| R-free | 0.26850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2kq9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.300 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.139 | 0.417 |
| Number of reflections | 50322 | |
| <I/σ(I)> | 14.7 | 3.2 |
| Completeness [%] | 92.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | 12% isopropanol, 0.2 M calcium acetate, 0.1 M MES, 0.1 M LiCl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | isopropanol | ||
| 2 | 1 | 1 | calcium acetate | ||
| 3 | 1 | 1 | MES | ||
| 4 | 1 | 1 | LiCl | ||
| 5 | 1 | 2 | isopropanol | ||
| 6 | 1 | 2 | calcium acetate | ||
| 7 | 1 | 2 | MES | ||
| 8 | 1 | 2 | LiCl |
