2QKB

Human RNase H catalytic domain mutant D210N in complex with 20-mer RNA/DNA hybrid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Wavelength(s)	1.000
Spacegroup name	P 32 2 1
Unit cell lengths	109.991, 109.991, 67.253
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.400
Rwork	0.208
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2qk9
RMSD bond length	0.013
RMSD bond angle	1.742
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.060	0.452
Number of reflections	16949
<I/σ(I)>	35.8	2.7
Completeness [%]	90.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	294	15% PEG 3350, 0.1 M LiSO4, 0.1 M Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 3350
2	1	1	LiSO4
3	1	1	Tris
4	1	2	PEG 3350
5	1	2	LiSO4
6	1	2	Tris