2QIS
Crystal structure of human farnesyl pyrophosphate synthase T210S mutant bound to risedronate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-26 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97956 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 111.470, 111.470, 67.603 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.920 - 1.800 |
| R-factor | 0.17926 |
| Rwork | 0.178 |
| R-free | 0.20593 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1zw5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.255 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.3.0037) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.566 |
| Number of reflections | 39744 | |
| <I/σ(I)> | 14.9 | 2.4 |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 0.20 M NH4Cl, 20.0% PEG 6000, 10.0% Ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
