2QHX
Structure of Pteridine Reductase from Leishmania major complexed with a ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.499, 104.231, 136.865 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.600 - 2.610 |
| Rwork | 0.183 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.129 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.600 | 2.670 |
| High resolution limit [Å] | 2.610 | 2.610 |
| Number of reflections | 40483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 11-14% PEG 5000, 0.1M SODIUM ACETATE, 0.04-0.14M CALCIUM ACETATE, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 293K, pH 5.50 |
