2QFD
Crystal structure of the regulatory domain of human RIG-I with bound Hg
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00800, 1.00900, 1.01000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.150, 76.540, 137.900 |
| Unit cell angles | 90.00, 93.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.253 |
| Rwork | 0.241 |
| R-free | 0.27600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.490 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.870 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 106277 | |
| <I/σ(I)> | 14.36 | 3.04 |
| Completeness [%] | 98.1 | 94.4 |
| Redundancy | 3.88 | 3.76 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 10 | 293.15 | 0.1 M CHES pH 10.0, 18% w/v PEG 8000, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |
