2QEQ
Crystal structure of kunjin virus ns3 helicase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.129, 38.095, 148.113 |
Unit cell angles | 90.00, 96.98, 90.00 |
Refinement procedure
Resolution | 39.750 - 3.100 |
R-factor | 0.24738 |
Rwork | 0.243 |
R-free | 0.33058 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2bmf |
RMSD bond length | 0.007 |
RMSD bond angle | 0.991 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.153 | 0.609 |
Number of reflections | 17953 | |
<I/σ(I)> | 10.9 | 3.2 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 3.3 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000 10%, Na Acetate 0.2 M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |