2Q7R
Crystal structure of human FLAP with an iodinated analog of MK-591
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-01 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 180.660, 180.660, 139.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 4.000 |
| R-factor | 0.2682 |
| Rwork | 0.268 |
| R-free | 0.28110 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.720 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHARP |
| Refinement software | BUSTER-TNT (1.9.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 4.240 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.111 | |
| Number of reflections | 20007 | |
| <I/σ(I)> | 20.6 | 3.29 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 15.5 | 15.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 298 | 10% PEG 4000, 0.32 LiCl2, 1 mM TCEP, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 298K, pH 5.60 |
