2Q7K
The Androgen Receptor Prostate Cancer Mutant H874Y Ligand Binding Domain Bound with Testosterone and an AR 20-30 Peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.932, 66.117, 70.681 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.720 - 1.800 |
| R-factor | 0.18112 |
| Rwork | 0.180 |
| R-free | 0.20856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.067 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.720 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.420 | 0.299 |
| Number of reflections | 23750 | |
| <I/σ(I)> | 41.1 | 3.8 |
| Completeness [%] | 98.5 | 87.4 |
| Redundancy | 6.7 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
