2Q6W
The structure of HLA-DRA, DRB3*0101 (DR52a) with bound platelet integrin peptide associated with fetal and neonatal alloimmune thrombocytopenia
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 98 |
| Detector technology | CCD |
| Collection date | 2002-01-31 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.169, 92.169, 248.555 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.050 - 2.250 |
| R-factor | 0.214 |
| Rwork | 0.210 |
| R-free | 0.26543 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlh |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.478 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.308 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.120 | 0.360 |
| Number of reflections | 48049 | |
| <I/σ(I)> | 3.04 | |
| Completeness [%] | 94.0 | 55.7 |
| Redundancy | 15.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 297 | 10-20% PEG 8000, 0.1M sodium acetate, 10% (v/v) glycerol, 0.01M Cd2+, pH 4.4, VAPOR DIFFUSION, HANGING DROP, temperature 297.0K |
