2Q62
Crystal Structure of ArsH from Sinorhizobium meliloti
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 32-ID |
| Synchrotron site | APS |
| Beamline | 32-ID |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2005-07-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 42 |
| Unit cell lengths | 158.534, 158.534, 87.953 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.630 - 1.800 |
| R-factor | 0.175 |
| Rwork | 0.173 |
| R-free | 0.21200 |
| Structure solution method | SIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.652 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SnB |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.633 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.088 | 0.532 |
| Total number of observations | 227608 | |
| Number of reflections | 176155 | |
| <I/σ(I)> | 5.7 | 1.5 |
| Completeness [%] | 92.1 | 83.5 |
| Redundancy | 11.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 296 | 17% PEG 6000, 0.1 M calcium acetate, 0.1 M cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
