2Q3E
Structure of human UDP-glucose dehydrogenase complexed with NADH and UDP-glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 115.990, 184.132, 170.943 |
| Unit cell angles | 90.00, 109.23, 90.00 |
Refinement procedure
| Resolution | 49.090 - 2.000 |
| R-factor | 0.17343 |
| Rwork | 0.172 |
| R-free | 0.20521 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2o3j |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.234 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.089 | 0.636 |
| Number of reflections | 452865 | |
| <I/σ(I)> | 9.7 | 2 |
| Completeness [%] | 99.6 | 99.1 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 16% PEG 3350, 8% EG, 160 mM NaBr, 80 mM BTP., pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
