2PYJ
Phi29 DNA polymerase complexed with primer-template DNA and incoming nucleotide substrates (ternary complex)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-10 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 72.835, 114.667, 104.761 |
Unit cell angles | 90.00, 94.07, 90.00 |
Refinement procedure
Resolution | 41.070 - 2.030 |
R-factor | 0.19128 |
Rwork | 0.189 |
R-free | 0.23409 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | copy c of 1xhx without residues 359-394 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.171 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.100 |
High resolution limit [Å] | 2.030 | 2.030 |
Rmerge | 0.076 | 0.353 |
Number of reflections | 107450 | |
<I/σ(I)> | 16.3 | 2.4 |
Completeness [%] | 98.0 | 83.9 |
Redundancy | 3.5 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 100 mM sodium acetate, 200 mM ammonium acetate, 15% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | sodium acetate | ||
2 | 1 | 1 | ammonium acetate | ||
3 | 1 | 1 | PEG 4000 | ||
4 | 1 | 2 | sodium acetate | ||
5 | 1 | 2 | ammonium acetate | ||
6 | 1 | 2 | PEG 4000 |