2PQA

Crystal Structure of Full-length Human RPA 14/32 Heterodimer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2000-08-26
Detector	MAR CCD 165 mm
Wavelength(s)	0.9794728, 0.979609, 0.999879
Spacegroup name	P 65
Unit cell lengths	63.377, 63.377, 272.641
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.500
R-factor	0.23091
Rwork	0.228
R-free	0.27981
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXS
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.580
High resolution limit [Å]	2.400	2.400
Number of reflections	24160
<I/σ(I)>	16.3	3.8
Completeness [%]	95.7	97.3
Redundancy	6	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine pH 9, 20% saturated ammonium sulfate, 10% acetonitrile, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 9.0
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine pH 9, 20% saturated ammonium sulfate, 10% acetonitrile, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 9.0
1	VAPOR DIFFUSION, HANGING DROP	9	293	0.1 M Bicine pH 9, 20% saturated ammonium sulfate, 10% acetonitrile, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 9.0