2PNL
Crystal structure of VP4 protease from infectious pancreatic necrosis virus (IPNV) in space group P1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X8C |
| Synchrotron site | NSLS |
| Beamline | X8C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.703, 69.262, 191.388 |
| Unit cell angles | 93.06, 95.03, 97.56 |
Refinement procedure
| Resolution | 182.570 - 2.210 |
| R-factor | 0.194 |
| Rwork | 0.190 |
| R-free | 0.26700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.754 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 182.570 | 2.290 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.082 | 0.280 |
| Number of reflections | 103935 | |
| <I/σ(I)> | 20.7 | 4.5 |
| Completeness [%] | 97.5 | 91.5 |
| Redundancy | 3.9 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl pH 8.5, 35% PEG 4000, 0.4M LiSO4, 0.4M Guanidine-HCl , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
