2PNC
Crystal Structure of Bovine Plasma Copper-Containing Amine Oxidase in Complex with Clonidine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-10 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97984 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.360, 131.956, 134.159 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.110 - 2.400 |
| R-factor | 0.23774 |
| Rwork | 0.237 |
| R-free | 0.25227 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tu5 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.877 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.129 | 0.407 |
| Number of reflections | 47787 | |
| <I/σ(I)> | 3.7 | 1.5 |
| Completeness [%] | 86.8 | 82.1 |
| Redundancy | 3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.4 | 293 | KH2PO4 (0.2 M, pH 7.4) and PEG 3350 (20%, w/v), VAPOR DIFFUSION, temperature 293K |
