2PMW

The Crystal Structure of Proprotein convertase subtilisin kexin type 9 (PCSK9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	63.106, 70.709, 150.693
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.300
Rwork	0.196
R-free	0.23200
Structure solution method	MIRAS
RMSD bond length	0.005
RMSD bond angle	0.841
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	30097
<I/σ(I)>	10.8	2.2
Completeness [%]	97.3	81.7
Redundancy	4.9	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1 M Tris (pH 7.8) 0.2 M (NH4)2SO4 13% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K