2PMP
Structure of 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase from the isoprenoid biosynthetic pathway of Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0358 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 135.795, 135.795, 135.795 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.600 - 2.300 |
| R-factor | 0.2 |
| Rwork | 0.198 |
| R-free | 0.25100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gx1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.507 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.600 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 9624 | |
| <I/σ(I)> | 7 | |
| Completeness [%] | 100.0 | 89.67 |
| Redundancy | 19.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 18% PEG3350, 100mM MES, 200mM Ammonium Dihydrogen Phosphate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
